The effect of altered lignin composition on mechanical properties of CINNAMYL ALCOHOL DEHYDROGENASE (CAD) deficient poplars.
Identifieur interne : 000C37 ( Main/Exploration ); précédent : 000C36; suivant : 000C38The effect of altered lignin composition on mechanical properties of CINNAMYL ALCOHOL DEHYDROGENASE (CAD) deficient poplars.
Auteurs : Merve Özparpucu [Suisse] ; Notburga Gierlinger [Autriche] ; Ingo Burgert [Suisse] ; Rebecca Van Acker [Belgique] ; Ruben Vanholme [Belgique] ; Wout Boerjan [Belgique] ; Gilles Pilate [France] ; Annabelle Déjardin [France] ; Markus Rüggeberg [Suisse]Source :
- Planta [ 1432-2048 ] ; 2018.
Descripteurs français
- KwdFr :
- Alcohol oxidoreductases (déficit), Analyse spectrale Raman (MeSH), Diffraction des rayons X (MeSH), Lignine (métabolisme), Lignine (physiologie), Microfibrilles (métabolisme), Microfibrilles (physiologie), Populus (métabolisme), Populus (physiologie), Résistance à la traction (MeSH), Spectroscopie infrarouge à transformée de Fourier (MeSH).
- MESH :
- déficit : Alcohol oxidoreductases.
- métabolisme : Lignine, Microfibrilles, Populus.
- physiologie : Lignine, Microfibrilles, Populus.
- Analyse spectrale Raman, Diffraction des rayons X, Résistance à la traction, Spectroscopie infrarouge à transformée de Fourier.
English descriptors
- KwdEn :
- Alcohol Oxidoreductases (deficiency), Lignin (metabolism), Lignin (physiology), Microfibrils (metabolism), Microfibrils (physiology), Populus (metabolism), Populus (physiology), Spectroscopy, Fourier Transform Infrared (MeSH), Spectrum Analysis, Raman (MeSH), Tensile Strength (MeSH), X-Ray Diffraction (MeSH).
- MESH :
- chemical , deficiency : Alcohol Oxidoreductases.
- chemical , metabolism : Lignin.
- chemical , physiology : Lignin.
- metabolism : Microfibrils, Populus.
- physiology : Microfibrils, Populus.
- Spectroscopy, Fourier Transform Infrared, Spectrum Analysis, Raman, Tensile Strength, X-Ray Diffraction.
Abstract
MAIN CONCLUSION
CAD-deficient poplars enabled studying the influence of altered lignin composition on mechanical properties. Severe alterations in lignin composition did not influence the mechanical properties. Wood represents a hierarchical fiber-composite material with excellent mechanical properties. Despite its wide use and versatility, its mechanical behavior has not been entirely understood. It has especially been challenging to unravel the mechanical function of the cell wall matrix. Lignin engineering has been a useful tool to increase the knowledge on the mechanical function of lignin as it allows for modifications of lignin content and composition and the subsequent studying of the mechanical properties of these transgenics. Hereby, in most cases, both lignin composition and content are altered and the specific influence of lignin composition has hardly been revealed. Here, we have performed a comprehensive micromechanical, structural, and spectroscopic analysis on xylem strips of transgenic poplar plants, which are downregulated for cinnamyl alcohol dehydrogenase (CAD) by a hairpin-RNA-mediated silencing approach. All parameters were evaluated on the same samples. Raman microscopy revealed that the lignin of the hpCAD poplars was significantly enriched in aldehydes and reduced in the (relative) amount of G-units. FTIR spectra indicated pronounced changes in lignin composition, whereas lignin content was not significantly changed between WT and the hpCAD poplars. Microfibril angles were in the range of 18°-24° and were not significantly different between WT and transgenics. No significant changes were observed in mechanical properties, such as tensile stiffness, ultimate stress, and yield stress. The specific findings on hpCAD poplar allowed studying the specific influence of lignin composition on mechanics. It can be concluded that the changes in lignin composition in hpCAD poplars did not affect the micromechanical tensile properties.
DOI: 10.1007/s00425-017-2828-z
PubMed: 29270675
Affiliations:
- Autriche, Belgique, France, Suisse
- Centre-Val de Loire, Province de Flandre-Orientale, Région Centre, Région flamande, Vienne (Autriche)
- Gand, Orléans, Vienne (Autriche)
- Université de Gand
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The effect of altered lignin composition on mechanical properties of CINNAMYL ALCOHOL DEHYDROGENASE (CAD) deficient poplars.</title><author><name sortKey="Ozparpucu, Merve" sort="Ozparpucu, Merve" uniqKey="Ozparpucu M" first="Merve" last="Özparpucu">Merve Özparpucu</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Gierlinger, Notburga" sort="Gierlinger, Notburga" uniqKey="Gierlinger N" first="Notburga" last="Gierlinger">Notburga Gierlinger</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Biophysics, University of Natural Resources and Life Sciences Vienna, 1190, Vienna, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute for Biophysics, University of Natural Resources and Life Sciences Vienna, 1190, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Burgert, Ingo" sort="Burgert, Ingo" uniqKey="Burgert I" first="Ingo" last="Burgert">Ingo Burgert</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf</wicri:regionArea><wicri:noRegion>Dübendorf</wicri:noRegion></affiliation></author><author><name sortKey="Van Acker, Rebecca" sort="Van Acker, Rebecca" uniqKey="Van Acker R" first="Rebecca" last="Van Acker">Rebecca Van Acker</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>VIB Center for Plant Systems Biology, 9052, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>VIB Center for Plant Systems Biology, 9052, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>VIB Center for Plant Systems Biology, 9052, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name><affiliation wicri:level="3"><nlm:affiliation>AGPF, INRA, 45075, Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>AGPF, INRA, 45075, Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Dejardin, Annabelle" sort="Dejardin, Annabelle" uniqKey="Dejardin A" first="Annabelle" last="Déjardin">Annabelle Déjardin</name><affiliation wicri:level="3"><nlm:affiliation>AGPF, INRA, 45075, Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>AGPF, INRA, 45075, Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Ruggeberg, Markus" sort="Ruggeberg, Markus" uniqKey="Ruggeberg M" first="Markus" last="Rüggeberg">Markus Rüggeberg</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland. mrueggeberg@ethz.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf, Switzerland. mrueggeberg@ethz.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf</wicri:regionArea><wicri:noRegion>Dübendorf</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29270675</idno><idno type="pmid">29270675</idno><idno type="doi">10.1007/s00425-017-2828-z</idno><idno type="wicri:Area/Main/Corpus">001025</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001025</idno><idno type="wicri:Area/Main/Curation">001025</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001025</idno><idno type="wicri:Area/Main/Exploration">001025</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The effect of altered lignin composition on mechanical properties of CINNAMYL ALCOHOL DEHYDROGENASE (CAD) deficient poplars.</title><author><name sortKey="Ozparpucu, Merve" sort="Ozparpucu, Merve" uniqKey="Ozparpucu M" first="Merve" last="Özparpucu">Merve Özparpucu</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Gierlinger, Notburga" sort="Gierlinger, Notburga" uniqKey="Gierlinger N" first="Notburga" last="Gierlinger">Notburga Gierlinger</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Biophysics, University of Natural Resources and Life Sciences Vienna, 1190, Vienna, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute for Biophysics, University of Natural Resources and Life Sciences Vienna, 1190, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Burgert, Ingo" sort="Burgert, Ingo" uniqKey="Burgert I" first="Ingo" last="Burgert">Ingo Burgert</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf</wicri:regionArea><wicri:noRegion>Dübendorf</wicri:noRegion></affiliation></author><author><name sortKey="Van Acker, Rebecca" sort="Van Acker, Rebecca" uniqKey="Van Acker R" first="Rebecca" last="Van Acker">Rebecca Van Acker</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>VIB Center for Plant Systems Biology, 9052, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>VIB Center for Plant Systems Biology, 9052, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>VIB Center for Plant Systems Biology, 9052, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name><affiliation wicri:level="3"><nlm:affiliation>AGPF, INRA, 45075, Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>AGPF, INRA, 45075, Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Dejardin, Annabelle" sort="Dejardin, Annabelle" uniqKey="Dejardin A" first="Annabelle" last="Déjardin">Annabelle Déjardin</name><affiliation wicri:level="3"><nlm:affiliation>AGPF, INRA, 45075, Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>AGPF, INRA, 45075, Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Ruggeberg, Markus" sort="Ruggeberg, Markus" uniqKey="Ruggeberg M" first="Markus" last="Rüggeberg">Markus Rüggeberg</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland. mrueggeberg@ethz.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf, Switzerland. mrueggeberg@ethz.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf</wicri:regionArea><wicri:noRegion>Dübendorf</wicri:noRegion></affiliation></author></analytic><series><title level="j">Planta</title><idno type="eISSN">1432-2048</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alcohol Oxidoreductases (deficiency)</term><term>Lignin (metabolism)</term><term>Lignin (physiology)</term><term>Microfibrils (metabolism)</term><term>Microfibrils (physiology)</term><term>Populus (metabolism)</term><term>Populus (physiology)</term><term>Spectroscopy, Fourier Transform Infrared (MeSH)</term><term>Spectrum Analysis, Raman (MeSH)</term><term>Tensile Strength (MeSH)</term><term>X-Ray Diffraction (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alcohol oxidoreductases (déficit)</term><term>Analyse spectrale Raman (MeSH)</term><term>Diffraction des rayons X (MeSH)</term><term>Lignine (métabolisme)</term><term>Lignine (physiologie)</term><term>Microfibrilles (métabolisme)</term><term>Microfibrilles (physiologie)</term><term>Populus (métabolisme)</term><term>Populus (physiologie)</term><term>Résistance à la traction (MeSH)</term><term>Spectroscopie infrarouge à transformée de Fourier (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Alcohol Oxidoreductases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Alcohol oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Microfibrils</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Microfibrilles</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Lignine</term><term>Microfibrilles</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Microfibrils</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Spectroscopy, Fourier Transform Infrared</term><term>Spectrum Analysis, Raman</term><term>Tensile Strength</term><term>X-Ray Diffraction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse spectrale Raman</term><term>Diffraction des rayons X</term><term>Résistance à la traction</term><term>Spectroscopie infrarouge à transformée de Fourier</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>MAIN CONCLUSION</b></p><p>CAD-deficient poplars enabled studying the influence of altered lignin composition on mechanical properties. Severe alterations in lignin composition did not influence the mechanical properties. Wood represents a hierarchical fiber-composite material with excellent mechanical properties. Despite its wide use and versatility, its mechanical behavior has not been entirely understood. It has especially been challenging to unravel the mechanical function of the cell wall matrix. Lignin engineering has been a useful tool to increase the knowledge on the mechanical function of lignin as it allows for modifications of lignin content and composition and the subsequent studying of the mechanical properties of these transgenics. Hereby, in most cases, both lignin composition and content are altered and the specific influence of lignin composition has hardly been revealed. Here, we have performed a comprehensive micromechanical, structural, and spectroscopic analysis on xylem strips of transgenic poplar plants, which are downregulated for cinnamyl alcohol dehydrogenase (CAD) by a hairpin-RNA-mediated silencing approach. All parameters were evaluated on the same samples. Raman microscopy revealed that the lignin of the hpCAD poplars was significantly enriched in aldehydes and reduced in the (relative) amount of G-units. FTIR spectra indicated pronounced changes in lignin composition, whereas lignin content was not significantly changed between WT and the hpCAD poplars. Microfibril angles were in the range of 18°-24° and were not significantly different between WT and transgenics. No significant changes were observed in mechanical properties, such as tensile stiffness, ultimate stress, and yield stress. The specific findings on hpCAD poplar allowed studying the specific influence of lignin composition on mechanics. It can be concluded that the changes in lignin composition in hpCAD poplars did not affect the micromechanical tensile properties.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29270675</PMID><DateCompleted><Year>2018</Year><Month>08</Month><Day>29</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2048</ISSN><JournalIssue CitedMedium="Internet"><Volume>247</Volume><Issue>4</Issue><PubDate><Year>2018</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>The effect of altered lignin composition on mechanical properties of CINNAMYL ALCOHOL DEHYDROGENASE (CAD) deficient poplars.</ArticleTitle><Pagination><MedlinePgn>887-897</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00425-017-2828-z</ELocationID><Abstract><AbstractText Label="MAIN CONCLUSION" NlmCategory="UNASSIGNED">CAD-deficient poplars enabled studying the influence of altered lignin composition on mechanical properties. Severe alterations in lignin composition did not influence the mechanical properties. Wood represents a hierarchical fiber-composite material with excellent mechanical properties. Despite its wide use and versatility, its mechanical behavior has not been entirely understood. It has especially been challenging to unravel the mechanical function of the cell wall matrix. Lignin engineering has been a useful tool to increase the knowledge on the mechanical function of lignin as it allows for modifications of lignin content and composition and the subsequent studying of the mechanical properties of these transgenics. Hereby, in most cases, both lignin composition and content are altered and the specific influence of lignin composition has hardly been revealed. Here, we have performed a comprehensive micromechanical, structural, and spectroscopic analysis on xylem strips of transgenic poplar plants, which are downregulated for cinnamyl alcohol dehydrogenase (CAD) by a hairpin-RNA-mediated silencing approach. All parameters were evaluated on the same samples. Raman microscopy revealed that the lignin of the hpCAD poplars was significantly enriched in aldehydes and reduced in the (relative) amount of G-units. FTIR spectra indicated pronounced changes in lignin composition, whereas lignin content was not significantly changed between WT and the hpCAD poplars. Microfibril angles were in the range of 18°-24° and were not significantly different between WT and transgenics. No significant changes were observed in mechanical properties, such as tensile stiffness, ultimate stress, and yield stress. The specific findings on hpCAD poplar allowed studying the specific influence of lignin composition on mechanics. It can be concluded that the changes in lignin composition in hpCAD poplars did not affect the micromechanical tensile properties.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Özparpucu</LastName><ForeName>Merve</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gierlinger</LastName><ForeName>Notburga</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institute for Biophysics, University of Natural Resources and Life Sciences Vienna, 1190, Vienna, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burgert</LastName><ForeName>Ingo</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Van Acker</LastName><ForeName>Rebecca</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vanholme</LastName><ForeName>Ruben</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>Wout</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pilate</LastName><ForeName>Gilles</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>AGPF, INRA, 45075, Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Déjardin</LastName><ForeName>Annabelle</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>AGPF, INRA, 45075, Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rüggeberg</LastName><ForeName>Markus</ForeName><Initials>M</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-6966-8311</Identifier><AffiliationInfo><Affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland. mrueggeberg@ethz.ch.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Applied Wood Materials, EMPA, 8600, Dübendorf, Switzerland. mrueggeberg@ethz.ch.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>Y 728</GrantID><Agency>Austrian Science Fund FWF</Agency><Country>Austria</Country></Grant><Grant><GrantID>130039</GrantID><Agency>SBO project BIOLEUM</Agency><Country></Country></Grant><Grant><GrantID>311804</GrantID><Agency>MultiBioPro</Agency><Country></Country></Grant><Grant><GrantID>START-project SURFINPLANT Y-728-316</GrantID><Agency>Austrian Science Fund</Agency><Country></Country></Grant><Grant><GrantID>140894</GrantID><Agency>SBO-FISH project ARBOREF</Agency><Country></Country></Grant><Grant><GrantID>postdoctoral fellowship</GrantID><Agency>Research Foundation Flanders</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.-</RegistryNumber><NameOfSubstance UI="D000429">Alcohol Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.195</RegistryNumber><NameOfSubstance UI="C018656">cinnamyl alcohol dehydrogenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000429" MajorTopicYN="N">Alcohol Oxidoreductases</DescriptorName><QualifierName UI="Q000172" MajorTopicYN="Y">deficiency</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020894" MajorTopicYN="N">Microfibrils</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013059" MajorTopicYN="N">Spectrum Analysis, Raman</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013718" MajorTopicYN="N">Tensile Strength</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014961" MajorTopicYN="N">X-Ray Diffraction</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cell wall mechanics</Keyword><Keyword MajorTopicYN="N">Genetic modification</Keyword><Keyword MajorTopicYN="N">Lignin composition</Keyword><Keyword MajorTopicYN="N">Plant cell wall</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>09</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>12</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>8</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>12</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29270675</ArticleId><ArticleId IdType="doi">10.1007/s00425-017-2828-z</ArticleId><ArticleId IdType="pii">10.1007/s00425-017-2828-z</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Front Plant Sci. 2012 Aug 22;3:204</Citation><ArticleIdList><ArticleId IdType="pubmed">22936943</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Biol Sci. 2008 Oct 7;275(1648):2221-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18595839</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2008 Jun;11(3):278-85</Citation><ArticleIdList><ArticleId IdType="pubmed">18434238</ArticleId></ArticleIdList></Reference><Reference><Citation>J Phys Chem B. 2010 Jun 17;114(23):8009-21</Citation><ArticleIdList><ArticleId IdType="pubmed">20499919</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2017 Aug;91(3):480-490</Citation><ArticleIdList><ArticleId IdType="pubmed">28440915</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1996 Dec;112(4):1479-1490</Citation><ArticleIdList><ArticleId IdType="pubmed">12226459</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mech Behav Biomed Mater. 2015 Feb;42:198-206</Citation><ArticleIdList><ArticleId IdType="pubmed">25498207</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2015 Sep 15;87(18):9436-42</Citation><ArticleIdList><ArticleId IdType="pubmed">26291845</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2003;54:519-46</Citation><ArticleIdList><ArticleId IdType="pubmed">14503002</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomacromolecules. 2010 Sep 13;11(9):2359-65</Citation><ArticleIdList><ArticleId IdType="pubmed">20831275</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2002 May;215(1):33-40</Citation><ArticleIdList><ArticleId IdType="pubmed">12012239</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2014 Jun 30;5:306</Citation><ArticleIdList><ArticleId IdType="pubmed">25071792</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2017 Nov;175(3):1018-1039</Citation><ArticleIdList><ArticleId IdType="pubmed">28878036</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1999 Jan;119(1):153-64</Citation><ArticleIdList><ArticleId IdType="pubmed">9880356</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):845-50</Citation><ArticleIdList><ArticleId IdType="pubmed">24379366</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Genet. 2010;44:337-63</Citation><ArticleIdList><ArticleId IdType="pubmed">20809799</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2016 Jul 07;9:139</Citation><ArticleIdList><ArticleId IdType="pubmed">27390589</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2007 Jul;25(7):759-61</Citation><ArticleIdList><ArticleId IdType="pubmed">17572667</ArticleId></ArticleIdList></Reference><Reference><Citation>J Struct Biol. 2013 Sep;183(3):419-428</Citation><ArticleIdList><ArticleId IdType="pubmed">23867392</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2002 Jun;20(6):607-12</Citation><ArticleIdList><ArticleId IdType="pubmed">12042866</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2012 Mar;109(3):647-56</Citation><ArticleIdList><ArticleId IdType="pubmed">22012706</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1999 Aug;17(8):808-12</Citation><ArticleIdList><ArticleId IdType="pubmed">10429249</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2011 Mar;189(4):1096-109</Citation><ArticleIdList><ArticleId IdType="pubmed">21158867</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2012 Sep;7(9):1694-708</Citation><ArticleIdList><ArticleId IdType="pubmed">22918387</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Autriche</li><li>Belgique</li><li>France</li><li>Suisse</li></country><region><li>Centre-Val de Loire</li><li>Province de Flandre-Orientale</li><li>Région Centre</li><li>Région flamande</li><li>Vienne (Autriche)</li></region><settlement><li>Gand</li><li>Orléans</li><li>Vienne (Autriche)</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><country name="Suisse"><noRegion><name sortKey="Ozparpucu, Merve" sort="Ozparpucu, Merve" uniqKey="Ozparpucu M" first="Merve" last="Özparpucu">Merve Özparpucu</name></noRegion><name sortKey="Burgert, Ingo" sort="Burgert, Ingo" uniqKey="Burgert I" first="Ingo" last="Burgert">Ingo Burgert</name><name sortKey="Burgert, Ingo" sort="Burgert, Ingo" uniqKey="Burgert I" first="Ingo" last="Burgert">Ingo Burgert</name><name sortKey="Ruggeberg, Markus" sort="Ruggeberg, Markus" uniqKey="Ruggeberg M" first="Markus" last="Rüggeberg">Markus Rüggeberg</name><name sortKey="Ruggeberg, Markus" sort="Ruggeberg, Markus" uniqKey="Ruggeberg M" first="Markus" last="Rüggeberg">Markus Rüggeberg</name></country><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Gierlinger, Notburga" sort="Gierlinger, Notburga" uniqKey="Gierlinger N" first="Notburga" last="Gierlinger">Notburga Gierlinger</name></region></country><country name="Belgique"><region name="Région flamande"><name sortKey="Van Acker, Rebecca" sort="Van Acker, Rebecca" uniqKey="Van Acker R" first="Rebecca" last="Van Acker">Rebecca Van Acker</name></region><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><name sortKey="Van Acker, Rebecca" sort="Van Acker, Rebecca" uniqKey="Van Acker R" first="Rebecca" last="Van Acker">Rebecca Van Acker</name><name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name><name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name></country><country name="France"><region name="Centre-Val de Loire"><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name></region><name sortKey="Dejardin, Annabelle" sort="Dejardin, Annabelle" uniqKey="Dejardin A" first="Annabelle" last="Déjardin">Annabelle Déjardin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C37 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000C37 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:29270675
|texte= The effect of altered lignin composition on mechanical properties of CINNAMYL ALCOHOL DEHYDROGENASE (CAD) deficient poplars.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29270675" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |